Live cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. Live cell imaging was pioneered in first decade of the 20th century. One of the first time-lapse microcinematographic films of cells ever made was made by Julius Ries, showing the fertilization and development of the sea urchin egg. Since then, several microscopy methods have been developed which allow researchers to study living cells in greater detail with less effort. A newer type of imaging utilizing quantum dots have been used as they are shown to be more stable.
Video Live cell imaging
Phase contrast microscopy
Before the introduction of the phase contrast microscope it was difficult to observe living cells. As living cells are translucent they must be stained to be visible in a traditional light microscope. Unfortunately, the process of staining cells generally kill the cells. With the invention of the phase contrast microscopy it became possible to observe unstained living cells in detail. After its introduction in the 1940s, live cell imaging rapidly became popular using phase contrast microscopy. The phase contrast microscope was popularized through a series of time-lapse movies (Video 1), recorded using a photographic film camera. Its inventor, Frits Zernike, was awarded the Nobel Prize in 1953. Other later phase contrast techniques used to observe unstained cells are Hoffman modulation and differential interference contrast microscopy.
Maps Live cell imaging
Fluorescent microscopy
Phase contrast microscopy does not have the capacity to observe specific proteins or other organic chemical compounds which form the complex machinery of a cell. Synthetic and organic fluorescent stains have therefore been developed to label such compounds, making them observable by fluorescent microscopy (Video 2). Fluorescent stains are, however, phototoxic, invasive and bleach when observed. This limits their use when observing living cells over extended periods of time. Non-invasive phase contrast techniques are therefore often used as a vital complement to fluorescent microscopy in live cell imaging applications.
Quantitative phase contrast microscopy
As a result of the rapid increase in pixel density of digital image sensors, quantitative phase contrast microscopy has emerged as an alternative microscopy method for live cell imaging. Quantitative phase contrast microscopy has an advantage over fluorescent and phase contrast microscopy in that it is both non-invasive and quantitative in its nature. Contrary to phase contrast images, quantitative phase contrast images (Video 3) can be automatically processed to extract vast amount of dynamic cellular data from time-lapse image sequences.
Due to the narrow focal depth of conventional microscopy, live cell imaging is to a large extent currently limited to observing cells on a single plane. Most implementations of quantitative phase contrast microscopy allow for images to be created and focused at different focal planes from a single exposure. This opens up the future possibility of 3-dimensional live cell imaging by means of fluorescence techniques. A marker-free holographic technique that instead relies on complex deconvolution has been implemented and commercialized by www.Nanolive.com, which provides access to the non-invasive 3-dimensional imaging of live single cells.
See also
- Cytometry
- Quantitative phase imaging
- Time-lapse microscopy
- Live single-cell imaging
References
External links
- NanoLive -- Self-adjusting & holographic living cell tomography
- Florida State University -- Introduction to Live Cell Imaging Techniques
- Carl Zeiss Education in Microscopy and Digital Imaging -- Live Cell Imaging
Source of article : Wikipedia
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